Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.

316 – 330 of 5,722 results

316

MilliporeSigma™ Novagen™ Ekapture™ Agarose

Intended for rapid affinity-based capture.

Pricing & Availability
Specifications

Type	Agarose
Content And Storage	2°C to 8°C

317

MilliporeSigma™ Novagen™ S-protein Agarose

Retains specifically S·Tag™ fusion proteins.

Pricing & Availability

318

Cytiva Glutathione Sepharose™ High Performance Media

For high resolution purification of Glutathione S transferase (GST) tagged proteins

Pricing & Availability
Specifications

Particle Size	34 μm
Ligand Type	Glutathione
Flow Rate	<600 cm/hr.
Type	Chromatography Media
Content And Storage	4°C to 30°C, 20% Ethanol
Matrix	Highly crossed-linked agarose, 6%
Description	Glutathione Sepharose High Performance is an affinity resin recommended for purifying glutathione S-transferase (GST)-tagged proteins when high resolution is the priority.
pH Range	3 to 12
Binding Capacity	>Approx. 10 mg GST-tagged protein/mL resin
Support Type	6% Highly Cross-linked Agarose

319

Cytiva GSTrap™ 4B Prepacked Columns: 7 x 25mm

Use for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins. Cytiva Life Sciences™ GSTrap™ 4B Prepacked Columns: 7 x 25mm are prepacked with Glutathione Sepharose™ 4B

Pricing & Availability
Specifications

Ligand Type	Glutathione
Flow Rate	<4 mL/min.
Type	Pre-packed Column
Content And Storage	4°C to 30°C, 20% Ethanol
Diameter (English) Inner	0.27 in.
Bed Volume	1 mL
Description	GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.
Max. Operating Pressure	5 bar (0.5 MPa, 70 psi)
pH Range	4 to 13
Diameter (Metric) Inner	7 mm
Material	Polypropylene
For Use With (Application)	GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function.
Support Type	4% Highly Cross-linked Agarose

320

Cytiva MBPTrap™ HP Prepacked Columns

Yield highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes. Cytiva Life Sciences™ MBPTrap™ HP Prepacked Columns are prepacked with Dextrin Sepharose™ High Performance.

Pricing & Availability
Specifications

Buffer	Aqueous Buffer
Height (Metric)	25mm
Content And Storage	2° to 8°C
Bed Volume	1mL
pH Range	>7
Column Type	Ion Exchange Column
Material	Polypropylene
For Use With (Application)	Purification of MBP tagged Proteins
Max. Pressure	3 bar
Particle Size	34μm
Ligand Type	Dextrin
Product Type	Dextrin Sepharose HP prepacked column
Support Type	6% Highly Cross-linked Agarose
Height (English)	0.98 in.

321

Cytiva HiTrap Capto MMC Columns

HiTrap Capto MMC is prepacked with Capto MMC, a multimodal BioProcess ion exchange medium. The multimodal functionality gives a different selectivity compared with traditional ion exchangers and it binds proteins at high or low ionic strength.

Pricing & Availability
Specifications

Particle Size	75μm
Buffer	Aqueous Buffer
Height (Metric)	25mm
Content And Storage	4° to 30°C
pH Range	2 to 12
Product Type	HiTrap Capto MMC column
Column Type	Affinity Column
Material	Polypropylene
For Use With (Application)	Bind Proteins at the conductivity of the feed material and to solve specific purification challenges
Support Type	Highly Cross-linked Agarose
Height (English)	0.98 in.

322

Cytiva GSTrap™ HP Columns

Use to provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins. Cytiva Life Sciences™ GSTrap™ HP Columns are prepacked with Glutathione Sepharose™ High Performance medium.

Pricing & Availability
Specifications

Particle Size	34 μm
Ligand Type	Glutathione
Type	Pre-packed Column
Content And Storage	4°C to 30°C, 20% Ethanol
Description	GSTrap HP columns are prepacked with Glutathione Sepharose High Performance resin and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.
Max. Operating Pressure	5 bar (0.5 MPa, 70 psi)
pH Range	3 to 12
Material	Polypropylene
For Use With (Application)	Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins
Support Type	4% Highly Cross-linked Agarose

323

Cytiva Glutathione Sepharose™ 4 Fast Flow Media

Single-step purification of GST-tagged proteins and other glutathione S-transferase or glutathione-dependent proteins. Glutathione Sepharose 4 Fast Flow Media is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.

Pricing & Availability
Specifications

Particle Size	90 μm
Ligand Type	Glutathione and 10-carbon linker arm
Flow Rate	<450 cm/hr.
Type	Chromatography Media
Content And Storage	4°C to 30°C, 20% Ethanol
Matrix	Highly cross-linked 4% agarose
Kit Contents	10 Carbon Spacer Arm, 4% Agarose, 90 μm Particle Size
Description	Glutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins in a batch mode or when scale up is needed.
Max. Operating Pressure	base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
pH Range	3 to 12
Binding Capacity	>10 mg/mL
Support Type	4% Highly Cross-linked Agarose

324

Cytiva SOURCE™ Tricorn™ Columns for IEX

Source 15Q 4.6/100 PE is a strong anion exchanger prepacked in a Tricorn column for intermediate lab-scale purification and large-scale polishing of biomolecules with ion exchange chromatography.

Pricing & Availability
Specifications

Buffer	All common ion exchange Buffers
Height (Metric)	100mm
Content And Storage	4° to 30°C
Diameter (English) Inner	0.18 in.
Bed Volume	1.7mL
Column Type	Ion Exchange Column
Diameter (Metric) Inner	4.6mm
Quantity	1 x 1.7mL
Material	PEEK
For Use With (Application)	High performance ion exchange chromato graphy of Proteins, Peptides, Polynucleotides and other biomolecules
Max. Pressure	40 bar
Particle Size	15μm
Flow Rate	<5 mL/min.
Support Type	Polystyrene/Divinylbenzene
Height (English)	3.93 in.

325

Cytiva Ni Sepharose™ High Performance Media

Precharged with Ni²⁺ for optimal purification of histidine-tagged proteins. Cytiva Lifescience™ Ni Sepharose™ High Performance Media shows high binding capacity and compatibility.

Pricing & Availability
Specifications

Particle Size	34μm
Content And Storage	4° to 30°C
pH Range	3 to 12
Product Type	Ni Sepharose HP chromato graphy media
dimMatrix	Ni-Sepharose
Support Type	6% Cross-linked Agarose

326

Thermo Scientific™ Pierce™ Glutathione Chromatography Cartridges

Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.

Pricing & Availability
Specifications

Stationary Phase	Glutathione
Type	Chromatography Cartridge
Target	GST-Tagged Protein
Content And Storage	Upon receipt store at 4–8°C. Do not freeze.
Protein Form	Recombinant
Form	Liquid
Column Type	Affinity, Agarose Resin
Product Line	Pierce

327

Thermo Scientific™ AminoLink™ Plus Coupling Resin

Covalently couple antibodies and other proteins via primary amines (-NH2) for affinity purification with these aldehyde-activated agarose beads.

Pricing & Availability
Specifications

Stationary Phase	Aldehyde
Type	Resin
Target	Antibody, Proteins through Primary Amine
Content And Storage	Store at 4°C.
Format	Bottle
Column Type	Affinity, Agarose Resin
Formulation	Aldehyde-activated 4% beaded agarose, slurried in water with sodium azide
Product Line	AminoLink

328

Cytiva His Mag Sepharose™ excel Chromatography Media

His Mag Sepharose excel magnetic beads are designed for small-scale purification and screening of histidine-tagged proteins secreted in eukaryotic cell culture.

Pricing & Availability
Specifications

Particle Size	63 μm
Type	Chromatography Media
Content And Storage	4°C to 30°C
pH Range	2 to 12
For Use With (Application)	Designed to simplify small-scale purification and screening of His-tagged Proteins secreted in eukaryotic cell culture, used for functional and structural studies research in academia, institutes and discovery Pharma
Support Type	Highly cross-linked spherical Agarose including Magnetite

329

Cytiva SOURCE™ 15Q

Strong anion exchanger with high chemical stability for wide range of working conditions with good resistance to cleaning conditions at high pH

Pricing & Availability
Specifications

Particle Size	15μm
Buffer	20mM N-methylpiperazine, 20mM Piperazine, 20mM Bis-Tris, 20mM Triethanolamine, 20mM Tris, 20mM Diethanolamine, 20mM Ethanolamine, Glycine, 20mM 1,3-diamino-propane, 20mM Piperidine
Ligand Type	Quaternary Ammonium
Flow Rate	1800 cm/hr.
Height (Metric)	100mm
Content And Storage	4° to 30°C
pH Range	2 to 12
Product Type	Source 15Q chromato graphy media
dimMatrix	15Q Chromatography Media
For Use With (Application)	Polishing steps in both research and industrial applications
Support Type	Polystyrene/Divinylbenzene
Max. Pressure	5 bar
Height (English)	3.93 in.

330

Cytiva GST Gene Fusion System pGEX Vectors

Designed for inducible, high-level intracellular expression of genes or gene fragments as fusions with Schistosoma japonicum GST

Pricing & Availability
Specifications

Type	pGEX Vector
Quantity	25 μg
For Use With (Application)	Designed for inducible, high-level intracellular expression of genes or gene fragments as fusions with Schisto soma japonicum GST

Protein Purification Reagents and Kits

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MilliporeSigma™ Novagen™ S-protein Agarose GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Cytiva MBPTrap™ HP Prepacked Columns GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Cytiva Glutathione Sepharose™ 4 Fast Flow Media GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Cytiva Ni Sepharose™ High Performance Media GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Cytiva His Mag Sepharose™ excel Chromatography Media GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Cytiva GST Gene Fusion System pGEX Vectors GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More